CCR5 (phosphoserine 349) antibody

• Catalog Number: orb758953
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CCR5 (phosphoserine 349)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: E11/19
• Tested applications: ELISA, IF, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This antibody was raised by immunizing mice with a synthetic phosphopeptide (CEAPERA(pS)(pS)VYTR(pS)TGEQI(pS)VGL) corresponding to the C-terminal region of human CCR5.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CCR5 (phosphoserine 349)
• UniProt ID: P51681
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD195; C-C chemokine receptor type 5; C-C CKR-5; C
• Note: For research use only
• Application notes: The E11/19 antibody is recommended for for flow cytometric analysis, immunoblotting, and ELISA assay of CCR5 expression. It was also successfully utilized in the immunoprecipitation of CCR5 (Venuti et al., 2016). This antibody was demonstrated to detect pS349 CCR5 via Western blot when it was first activated to its phosphorylated form (Pollok-Kopp et al., 2003). Furthermore, E11/19 antibody was used to identify the localization of pS349 CCR5 in RBL cells via immunofluorescence (Pollok-Kopp et al., 2003). With other anti-CCR5 antibodies which react with nonphosporylated CCR5 (Ab01577) or CCR5 phosphorylated on different serine residues (Ab01579), it can be used to study dynamic regulation of CCR5 and of phosphorylation mechanisms.
• Expiration Date: 12 months from date of receipt.