CCR5 (phosphoserine 349) Antibody [E11/19], Rabbit IgG

• Catalog Number: orb1671599
• Category: Antibodies
• Description: CCR5 (phosphoserine 349) Antibody [E11/19], Rabbit IgG
• Target: CCR5
• Clonality: Recombinant
• Isotype: IgG kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: batch dependent
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: This antibody was raised by immunizing mice with a synthetic phosphopeptide (CEAPERA(pS)(pS)VYTR(pS)TGEQI(pS)VGL) corresponding to the C-terminal region of human CCR5.
• UniProt ID: P51681
• Tested applications: ELISA, IF, IP, WB
• Antibody Type: Primary Antibody
• Clone Number: E11/19
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD195, C-C chemokine receptor type 5, C-C CKR-5, C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-CCR5 (phosphoserine 349) antibody E11/19. Molt4 cell lysate (35 ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of E11/19 (orb1671599) at 0.1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescence staining of fixed U937 cells with anti-CCR5 (phosphoserine 349) antibody E11/19. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version of E11/19 (orb1671599) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671599- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the anti-CCR5 (phosphoserine 349) antibody E11/19. U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version of E11/19 (orb1671599 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.