CCNK monoclonal antibody (M01), clone 3B7-1B9

• Catalog Number: orb2291826
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant CCNK.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: CCNK (AAH15935, 1 a.a. ~ 354 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYDTLATGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEVMVLERILLQTIKFDLQVEHPYQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAVMYLAGRLCKFEIQEWTSKPMYRRWWEQFVQDVPVDVLEDICHQILDLYSQGKQQMPHHTPHQLQQPPSLQPTPQVPQVQQSQPSQSSEPSQPQQKDPLILLQGWACRQPATHLLPSPLEDSLLCPRPFPHPACLQLGGWGGQPG
• Tested applications: ELISA, WB
• Clone Number: 3B7-1B9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH15935

CCNK monoclonal antibody (M01), clone 3B7-1B9. Western Blot analysis of CCNK expression in PRC/PRF/5.

Detection limit for recombinant GST tagged CCNK is approximately 0.03 ng/ml as a capture antibody.

Western Blot analysis of CCNK expression in transfected 293T cell line by CCNK monoclonal antibody (M01), clone 3B7-1B9. Lane 1: CCNK transfected lysate (41 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of CCNK over-expressed 293 cell line, cotransfected with CCNK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CCNK monoclonal antibody (M01), clone 3B7-1B9 (Cat # orb2291826). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (64.68 KDa).