You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1272394 |
---|---|
Category | Antibodies |
Description | Ccl7 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, NeA, WB |
Reactivity | Mouse |
Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mMCP-3 (murine Macrophage/Monocyte chemotactic protein-3). |
Concentration | batch dependent |
Dilution range | Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of Murine MCP-3 (100.0 ng/mL), a concentration of 4.0 - 6.0 μg/mL of this antibody is required..ELISA:To detect mMCP-3 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMCP-3.Sandwich:To detect mMCP-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MCP-3as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3. Western Blot:To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | Ccl7 |
UniProt ID | Q03366 |
NCBI | Q03366 |
Storage | MCP-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | fic, marc, mcp3, MCP-3, Scya7, Fic, Mcp3, C-C moti Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
To detect mMCP-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine MCP-3 (orb1272393) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3.
To detect mMCP-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine MCP-3 (orb1272393) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3.
To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA, ICC, IF, IHC-P | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating