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Catalog Number | orb1272334 |
---|---|
Category | Antibodies |
Description | Ccl5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Rat |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat Rantes. |
Concentration | batch dependent |
Dilution range | ELISA:To detect Rat Rantes by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat Rantes. Sandwich:To detect Rat RANTES by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat RANTESas a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat RANTES.Western Blot:To detect Rat Rantes by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat Rantes is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | Ccl5 |
UniProt ID | P50231 |
NCBI | P50231 |
Storage | RANTES antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | Scya5, Rantes, Scya5, C-C motif chemokine 5, SIS-d Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
To detect Rat RANTES by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Rat RANTES (orb1272333) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat RANTES.
To detect Rat RANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat RANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat RANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat RANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained colchicine injected rat brain (somatosensory cortex) tissue. The primary antibody was incubated at 1.0 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
ELISA, ICC, IF, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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