CCL28 Antibody

• Catalog Number: orb1272639
• Category: Antibodies
• Description: CCL28 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human MEC. Human MEC specific antibody was purified by affinity chromatography employing an immobilized Human MEC matrix.
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: CCL28
• UniProt ID: Q9NRJ3
• NCBI: Q9NRJ3
• Storage: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: MEC, CCK1, SCYA28, C-C motif chemokine 28, Mucosae
• Note: For research use only
• Application notes: ELISA:Sandwich:To detect hMEC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with BIorbyt’s Biotinylated Anti-Human MEC (38-279) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC.Western Blot:To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect hMEC by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MEC (orb1272628) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC.

To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained CACO-2 cells. The primary antibody was incubated at 3.0 ug/ml overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary.