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Catalog Number | orb1272558 |
---|---|
Category | Antibodies |
Description | CCL26 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, NeA, WB |
Reactivity | Human |
Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant human Eotaxin-3. |
Concentration | batch dependent |
Dilution range | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of human eotaxin-3 (100.00 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required. ELISA:To detect human eotaxin-3 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant human eotaxin-3. Sandwich: To detect hEotaxin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Eotaxin-3as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin-3.Western Blot:To detect human eotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant human eotaxin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | CCL26 |
UniProt ID | Q9Y258 |
NCBI | Q9Y258 |
Storage | Eotaxin-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | IMAC, TSC-1, MIP-4a, SCYA26, MIP-4alpha, UNQ216/PR Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
To detect hEotaxin-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human Eotaxin-3 (orb1272557) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin-3.
To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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