CCL25 Antibody

• Catalog Number: orb1272252
• Category: Antibodies
• Description: CCL25 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1.2_4G1-1G4-1C9
• Tested applications: ELISA, NeA, WB
• Reactivity: Human
• Immunogen: The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hTECK as the immunizing antigen.
• Concentration: batch dependent
• Dilution range: Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/mL), a concentration of 10.0-20.0 μg/mL of this antibody is required.ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 300 pg/mL of recombinant human TECK when used with our biotinylated antigen affinity purified anti-human TECK (XP-5280Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot:To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 0.50-1.0 ng/lane, under non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: CCL25
• UniProt ID: O15444
• NCBI: O15444
• Storage: TECK lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
• Alternative names: Thymus expressed chemokine, Small inducible cytoki
• Note: For research use only
• Application notes: Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/mL), a concentration of 10.0-20.0 μg/mL of this antibody is required.ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 300 pg/mL of recombinant human TECK when used with our biotinylated antigen affinity purified anti-human TECK (XP-5280Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot:To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 0.50-1.0 ng/lane, under non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 2.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen and a Proteinase K antigen retrieval. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 2.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen and a Proteinase K antigen retrieval. Optimal concentrations and conditions may vary.