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Catalog Number | orb1272261 |
---|---|
Category | Antibodies |
Description | CCL24 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1.1_6C7-2C5 |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human Eotaxin-2 as the immunizing antigen. |
Concentration | batch dependent |
Dilution range | ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with our biotinylated antigen affinity purified anti-human Eotaxin-2 (XP-5126Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL.Western blot:To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | CCL24 |
UniProt ID | O00175 |
NCBI | O00175 |
Storage | Eotaxin-2 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted. |
Alternative names | MPIF2, SCYA24, C-C motif chemokine 24, CK-beta-6, Read more... |
Note | For research use only |
Application notes | ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with our biotinylated antigen affinity purified anti-human Eotaxin-2 (XP-5126Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL.Western blot:To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with antigen affinity purified anti-human Eotaxin-2 (orb1272559) as the detection antibody at a concentration of approximately 1.0-2.0 ug/ml.
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary.
ELISA, IHC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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