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Catalog Number | orb1272382 |
---|---|
Category | Antibodies |
Description | CCL22 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, NeA, WB |
Reactivity | Human |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMDC (human Macrophage-Derived Chemokine). |
Concentration | batch dependent |
Dilution range | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMDC (100 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required. ELISA:To detect hMDC by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMDC. Sandwich:To detect hMDC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MDCas a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMDC.Western Blot:To detect hMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | CCL22 |
UniProt ID | O00626 |
NCBI | O00626 |
Storage | MDC antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | MDC, ABCD-1, SCYA22, STCP-1, DC/B-CK, A-152E5.1, M Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
To detect hMDC by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human MDC (orb1272381) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMDC.
To detect hMDC by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMDC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hMDC by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMDC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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