CCL20 / MIP-3-Alpha Antibody

• Catalog Number: orb1538966
• Category: Antibodies
• Description: CCL20 / MIP-3-Alpha Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1,k
• Immunogen: E.coli derived recombinant Human MIP-3alpha.
• Dilution range: ELISA (2 - 4 µg/ml), IHC, IHC-P (10 µg/ml), WB (0.2 - 0.4 µg/ml)
• Conjugation: Unconjugated
• Target: CCL20 / MIP-3-Alpha
• Storage: Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from PBS. Lyophilized from PBS
• Alternative names: CCL20, Beta chemokine exodus-1, Beta-chemokine exo
• Note: For research use only
• Application notes: Resuspension: Centrifuge the vial before opening. Reconstitute in sterile water to 0.1 mg/ml.. Further information: Immunohistochemistry: LS-B7409 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human MIP-3alpha when used with biotinylated antigen affinity purified anti-Human MIP-3alpha (LS-C104612) as the detection antibody at a concentration of approximately 1-2 ug/ml. Western Blot: To detect Human MIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3alpha is 0.25-0.50 ng/lane, under non-reducing conditions.
• Expiration Date: 12 months from date of receipt.