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Catalog Number | orb1671450 |
---|---|
Category | Antibodies |
Description | CCL15 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hMIP-5. Anti-Human MIP-5 specific antibody was purified by affinity chromatography employing immobilized hMIP-5 matrix. |
Concentration | batch dependent |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | CCL15 |
UniProt ID | Q16663 |
NCBI | Q16663 |
Storage | Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | Macrophage Inflammatory Protein-5, CCL15, HCC-2, L Read more... |
Note | For research use only |
Application notes | Sandwich ELISA: To detect hMIP-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biorbyt’s Biotinylated Anti-Human MIP-5 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-5. Western Blot To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect hMIP-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-5 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-5.
To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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