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Catalog Number | orb1272670 |
---|---|
Category | Antibodies |
Description | CCL11 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1.3_17H09-02C06 |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | Eotaxin antibody is produced in BALB/c mice using highly pure (>98%) recombinant human Eotaxin as the immunizing antigen. |
Concentration | batch dependent |
Dilution range | ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 800 pg/mL of recombinant human Eotaxin when used with our biotinylated antigen affinity purified anti-human Eotaxin as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot:To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 0.5-1.0 ng/lane, under reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | CCL11 |
UniProt ID | P51671 |
NCBI | P51671 |
Storage | Eotaxin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted. |
Alternative names | SCYA11, Eotaxin, C-C motif chemokine 11SCYA11 Read more... |
Note | For research use only |
Application notes | ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 800 pg/mL of recombinant human Eotaxin when used with our biotinylated antigen affinity purified anti-human Eotaxin as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot:To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 0.5-1.0 ng/lane, under reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
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