CCCP

SKU: orb1300092

Description

CCCP is a mitochondrial uncoupler that dissipates the proton gradient, inhibiting oxidative phosphorylation. It is widely used in vitro to study mitochondrial function, cellular metabolism, and innate immunity, as it also suppresses the STING-TBK1-IRF3 signaling pathway.

Research Area

Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research, Neuroscience, Signal Transduction

Key Properties

• CAS Number: 555-60-2
• MW: 204.62
• Purity: 97.78%
• Formula: C₉H₅ClN₄
• SMILES: ClC1=CC=CC(N\N=C(/C#N)C#N)=C1
• Target: PTEN,Antibacterial,COX,STING,IFNAR,Apoptosis,OXPHOS,IκB/IKK,Mitochondrial Metabolism
• Solubility: Ethanol:5 mg/mL (24.44 mM);DMSO:252.5 mg/mL (1233.99 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:6 mg/mL (29.32 mM)

Bioactivity

• Target IC50:
  HepG2 cells:9.8 μM|HepG2 cells:7.2 μM|HepG2 cells:6 μM
• In Vivo:
  METHODS: To assay in vivo activity, CCCP (5 mg/kg) was administered as a single intraperitoneal injection to adult and aged C57BL/6J mice, and hearts were collected 12 h later. RESULTS: Under CCCP-treated conditions, more mitochondria bound to autophagic vesicles were found in the hearts of adult but not aged mice. METHODS: To assay in vivo activity, CCCP (1 mg/kg) was administered intraperitoneally to mt-Rosella mice once a day for three days. RESULTS: Mitochondrial fragments were readily visible on the hearts of CCCP-treated mice, and these fragments were trapped in lysosomes for degradation.CCCP induced mitochondrial autophagy.
• In Vitro:
  METHODS: Human cervical cancer cells HeLa were treated with CCCP (20 μM) for 30 min, and the effect on the mitochondria-labeled fluorescent dye DiOC6 was observed using live cell fluorescence microscopy. RESULTS: CCCP-induced loss of mitochondrial membrane potential resulted in efflux of DiOC6 from mitochondria into the cytoplasm and subsequent localization to punctate structures. METHODS: Mouse alveolar epithelial cells, MLE-12, were treated with CCCP (10-50 μM) for 2-18 h. The expression levels of the target proteins were examined by Western Blot. RESULTS: CCCP caused a dose- and time-dependent conversion of LC3-I to LC3-II.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CCCP, Apoptosis, Bacterial, Carbonyl Cyanide m-Chlorophenylhydrazone, Carbonyl cyanide 3-chlorophenylhydrazone, MPYS, IkB, IkB/IKK, IKK, IκB, inhibit, MitochondrialMetabolism, MITA, Mitochondrial Metabolism, ERIS, IFN-β, IFNAR, Inhibitor, Interferon-α/β receptor, Interferon-alpha/beta receptor, TBK1, Stimulator of Interferon Genes, STING, TMEM173

Compound Identifiers

PubChem CID

2603