CBX5 (phospho-S92) antibody

• Catalog Number: orb515267
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CBX5 (phospho-S92)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S92 of human HP1 alpha protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:50-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CBX5
• Entrez: 12419, 23468
• UniProt ID: Q61686, P45973
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: HP1A; Chromobox protein homolog 5; Antigen p25; He
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HP1 alpha (Phospho-S92) expression in PC3 (A), mouse heart (B), mouse brain (C), rat heart (D) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 25 kD)

Immunohistochemical analysis of HP1 alpha (Phospho-S92) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Direct ELISA antibody dose-response curve using Anti-HP1 alpha (Phospho-S92) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.