CBL Antibody

• Catalog Number: orb196248
• Category: Antibodies
• Description: CBL Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CBL recombinant protein (Position: A556-T906). Human CBL shares 84% amino acid (aa) sequence identity with mouse CBL.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 99633 MW
• UniProt ID: P22681
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: E3 ubiquitin-protein ligase CBL;6.3.2.-;Casitas B-
• Note: For research use only
• Application notes: WB: The detection limit for CBL is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-CBL antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CBL using anti-CBL antibody.Lane 1:human HeLa cell; 2:human MCF-7 cell; 3:human HepG2 cell; 4:human A549 cell; 5:rat RH35 cell; 6:mouse Ana-1 cell; 7:mouse NIH/3T3 cell.

IF analysis of CBL using anti-CBL antibody. CBL was detected in immunocytochemical section of U20S cells.

IHC analysis of CBL using anti-CBL antibody. CBL was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of CBL using anti-CBL antibody. CBL was detected in paraffin-embedded section of mouse brain tissues.

IHC analysis of CBL using anti-CBL antibody. CBL was detected in paraffin-embedded section of rat brain tissues.