CBFA2T2 antibody

• Catalog Number: orb341222
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CBFA2T2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human MTGR1
• Dilution range: WB: 1:500-1:2000, IF/ICC: 1:50-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CBFA2T2
• Entrez: 9139, 12396
• UniProt ID: O70374, O43439
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: EHT; MTGR1; Protein CBFA2T2; ETO homologous on chr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MTGR1 expression in Jurkat (A), HepG2 (B), mouse testis (C), mouse kidney (D), rat brain (E) whole cell lysates. (Predicted band size: 28; 63; 65; 67 kD; Observed band size: 67 kD)

Immunofluorescent analysis of MTGR1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.