Caveolin-2/CAV2 Antibody

• Catalog Number: orb215920
• Category: Antibodies
• Description: Caveolin-2/CAV2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Caveolin-2 recombinant protein (Position: M1-D162). Human Caveolin-2 shares 90% and 89% amino acid (aa) sequences identity with mouse and rat Caveolin-2, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunofluorescence, 2μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 18291 MW
• UniProt ID: P51636
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Caveolin-2;CAV2;
• Note: For research use only
• Application notes: WB: The detection limit for Caveolin-2 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-Caveolin-2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Caveolin-2 using anti-Caveolin-2 antibody.Lane 1:human HT1080 cell; 2:human U20S cell; 3:human Caco-2 cell; 4:human HeLa cell;5:human A549 cell;6:rat lung tissue;7:mouse lung tissue.

IF analysis of Caveolin-2 using anti-Caveolin-2 antibody. Caveolin-2 was detected in paraffin-embedded section of human rectal cancer tissue.

IF analysis of Caveolin-2 using anti-Caveolin-2 antibody. Caveolin-2 was detected in immunocytochemical section of A549 cells.

IHC analysis of Caveolin-2 using anti-Caveolin-2 antibody. Caveolin-2 was detected in paraffin-embedded section of human rectal cancer tissue.

IHC analysis of Caveolin-2 using anti-Caveolin-2 antibody. Caveolin-2 was detected in paraffin-embedded section of rat lung tissue.