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Catalog Number | orb1474555 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to Cathepsin B |
Species/Host | Rabbit |
Clonality | Monoclonal |
Tested applications | IF, WB |
Reactivity | Mouse, Rat |
Immunogen | Recombinant protein of mouse Cathepsin B |
Dilution range | WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) |
Form/Appearance | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin. |
Conjugation | Unconjugated |
Target | CTSB |
Entrez | 64529, 13030 |
UniProt ID | P00787, P10605 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin. |
Alternative names | CPSB; Cathepsin B; APP secretase; APPS; Cathepsin Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Cathepsin B expression in rat brain (A), C6 (B), NIH3T3 (C) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 44; 27; 24 kD)
Immunofluorescent analysis of Cathepsin B staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, IHC | |
Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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