Catalase Antibody

• Catalog Number: orb371659
• Category: Antibodies
• Description: Catalase Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Catalase recombinant protein (Position: E344-L527). Human Catalase shares 85.3% and 82.6% amino acid (aa) sequence identity with mouse and rat Catalase, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 59756 MW
• UniProt ID: P04040
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Catalase;1.11.1.6;CAT;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-Catalase antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Catalase using anti-Catalase antibody.Lane 1:human A549 cell; 2:human HL-60 cell; 3:human THP-1 cell; 4:human U937 cell; 5:human HepG2 cell; 6:human K562 cell; 7:human HCCT tissue; 8:human HCCP tissue.

WB analysis of Catalase using anti-Catalase antibody.Lane 1:rat liver tissue; 2:rat kidney tissue; 3:rat lung tissue; 4:rat RH35 cell; 5:mouse liver tissue; 6:mouse kidney tissue; 7:mouse lung tissue.

IF analysis of Catalase using anti-Catalase antibody.Catalase was detected in immunocytochemical section of A431 cell.

IHC analysis of Catalase using anti-Catalase antibody. Catalase was detected in a paraffin-embedded section of mouse liver tissue.

IHC analysis of Catalase using anti-Catalase antibody. Catalase was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of Catalase using anti-Catalase antibody. Catalase was detected in a paraffin-embedded section of rat liver tissue.