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Catalog Number | orb346850 |
---|---|
Category | Antibodies |
Description | Cat IgG F(ab')2 antibody (Peroxidase) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Feline |
Isotype | IgG |
Immunogen | Cat IgG F(ab')2 fragment |
Concentration | 2.0 mg/mL |
Dilution range | ELISA: 1:10,000 - 1:65,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000 |
Form/Appearance | Lyophilized |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Cat IgG, Cat IgG F(ab')2 and Cat Serum. No reaction was observed against Cat IgG F(c). |
Conjugation | HRP |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | Goat anti-Cat IgG F(ab')2 peroxidase Conjugated An Read more... |
Note | For research use only |
Application notes | Anti-Cat IgG F(ab')2 Peroxidase conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Cat IgG, anti-Cat IgG F(ab’)2 and anti-Cat Serum. No reaction was observed against anti-Cat IgG F(c) or anti-Pepsin. | |
Cat |
ELISA, IHC, WB | |
Feline | |
Rabbit | |
Polyclonal | |
HRP |
ELISA, IHC, WB | |
Feline | |
Rabbit | |
Polyclonal | |
HRP |
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