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Catalog Number | orb1294352 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Caspase 9 cleaved Asp315 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide corresponding to residues surrounding Asp315 of human Caspase 9 |
Dilution range | Western Blot 1:500 Immunofluorescence 1:300-1:400 Immunohistochemistry (Paraffin) 1:100-1:300 |
Conjugation | Unconjugated |
Target | Caspase-9 cleaved Asp315 |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-Caspase 9 cleaved Asp315 antibody - at 1/500 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294352 overnight at 4°C.
All lanes: Anti-Caspase 9 cleaved Asp315 antibody - at 1/500 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294352 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294352 Anti-Caspase 9 cleaved Asp315 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Caspase 9 cleaved Asp315 with orb1294352 at 1/200.
ELISA, ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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