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Catalog Number | orb76248 |
---|---|
Category | Antibodies |
Description | Caspase-8(p18)/CASP8 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase-8 (p18) (257-277aa NNHNFAKAREKVPKLHSIRDR). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Western blot, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 55391 MW |
UniProt ID | Q14790 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Caspase-8;CASP-8;3.4.22.61;Apoptotic cysteine prot Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Anti-Caspase-8 (p18) antibody, orb76248, Western blotting. All lanes: Anti Caspase-8 (p18) (orb76248) at 0.5ug/ml. Lane 1: A549 Whole Cell Lysate at 40ug, Lane 2: HELA Whole Cell Lysate at 40ug. Predicted bind size: 18KD. Observed bind size: 18KD.
Anti-Caspase-8 (p18) antibody, orb76248, IHC (P) IHC (P): Human Tonsil Tissue.
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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