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Catalog Number | orb763897 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Caspase-7 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Synthesized peptide derived from Caspase-7 . at AA range: 160-240 |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 34 |
Storage | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-CASP7 antibody, anti-MCH3 antibody, anti-Casp Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800 (diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute? Plasma Membrane Protein Isolation and Cell Fractionation Kit (SM-005, Inventbiotech, MN, USA).
Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1, Caspase-7 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, Caspase-7 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Caspase-7 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
ELISA, IF, IHC, IP, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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