CASPASE-3/7 Assay Kit

• Catalog Number: orb429518
• Category: Assays and Kits
• Description: Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. Their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.
• Target: CASPASE-3/7
• Conjugation: FAM
• Form/Appearance: Kit components: 1 vial of FAM-DEVD-FMK FLICA Reagent - lyophilized 10x Apoptosis Wash Buffer, 15 mL Fixative, 6 mL Propidium Iodide, 1 mL Hoechst 33342, 1 mL
• Tested applications: FACS, ICC, IF
• Dilution range: This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates
• Application notes: The caspase FLICA kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits
• Sensitivity: Max Ex/Em: Fluorophore - FAM Excitation Max (nm) - 494 Emission Max (nm) - 520
• Storage: Store the unopened kit and each unopened component at +4°C until the expiration date. Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20°C for 6 months protected from light and thawed no more than twice during that time.
• Note: For research use only