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Caspase-3 Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb704242

DispatchUsually dispatched within 5-10 working days
$ 200.00
Catalog Numberorb704242
CategoryAntibodies
DescriptionCaspase-3 Recombinant Rabbit Monoclonal Antibody
TargetCASP3
ClonalityRecombinant
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human Caspase-3
UniProt IDP42574
MW32 kDa
Tested applicationsIF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-3000, IHC-P=1:200-500, IHC-F=1:200-500, IF=1:200-500
Antibody TypeRecombinant Antibody
Clone Number5A3
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namescleaved Caspase 3; cleaved Caspase-3; APOPAIN; CAS
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NoteFor research use only
Caspase-3 Recombinant Rabbit Monoclonal Antibody

25 ug total protein per lane of various lysates probed with Caspase-3 monoclonal antibody, unconjugated (orb704242) at 1:3000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Caspase-3 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb704242) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Caspase-3 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Lymph, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (orb704242) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Caspase-3 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (orb704242) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Caspase-3 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb704242) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

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