Caspase 3 (Pro & Active Form) Antibody

• Catalog Number: orb2309393
• Category: Antibodies
• Description: Caspase 3 (Pro & Active Form) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CASP3/8446
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: Recombinant fragment (around aa1-200) of human CASP3 protein (exact sequence is proprietary)
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 32kDa
• Target: CASP3
• Entrez: 836
• UniProt ID: P42574
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: APOPAIN; Apoptosis related cysteine peptidase; CAS
• Note: For research use only
• Application notes: Positive Control: HL60 or Jurkat cells. Human liver, spleen, heart, liver or kidney.
• Expiration Date: 12 months from date of receipt.