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Catalog Number | orb345402 |
---|---|
Category | Antibodies |
Description | Caspase-2 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker. |
Concentration | 0.44 mg/mL |
Dilution range | ELISA: 1:1,500 - 1:6,000, WB: 1:750 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity purified antibody is directed against rat and mouse caspase-2. The antibody detects long and short forms of the protein. Antiserum was purified against the immunizing peptide. This antibody reacts with mouse and rat caspase-2, but not caspase-2 from human sources. Cross reactivity with caspase-2 from other sources has not been determined. Rat and mouse caspase-2 show 94% homology. There is no significant homology to the human form. |
Conjugation | Unconjugated |
UniProt ID | P55215 |
NCBI | AAD33684.1 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Caspase-2 Antibody, Apoptosis related Read more... |
Note | For research use only |
Application notes | This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kDa mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2. No reaction was observed against human caspase-2. Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2. A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of human 292 cell lysates using Caspase-2 antibody
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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