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Catalog Number | orb47989 |
---|---|
Category | Antibodies |
Description | Caspase-1(P10)/CASP1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-1 (P10) (249-262aa VFIGRLIEHMQEYA). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 45159 MW |
UniProt ID | P29466 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Caspase-1;CASP-1;3.4.22.36;Interleukin-1 beta conv Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Lane 1: NIH Cell Lysate, Lane 2: MCF-7 Cell Lysate, Lane 3: HELA Cell Lysate, Lane 4: SMMC Cell Lysate, Lane 5: HT1080 Cell Lysate, Lane 6: SW620 Cell Lysate, Lane 7: JURKAT Cell Lysate, Lane 8: RAJI Cell Lysate, Lane 9: CEM Cell
Immunohistochemical analysis of formalin-fixed paraffin embedded Human Mammary Cancer Tissue using Caspase 1 p10 antibody.
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