CASP8AP2 Antibody

• Catalog Number: orb1239799
• Category: Antibodies
• Description: CASP8AP2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: IgG
• Immunogen: FLASH antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human FLASH which differ from those of mouse by one amino acid.The immunogen is located within the last 50 amino acids of FLASH.
• Concentration: 1 mg/ml
• Dilution range: FLASH antibody can be used for detection of FLASH by Western blot at 0.5 μg/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 220 kDa
• Target: CASP8AP2
• UniProt ID: Q9UKL3
• NCBI: AF165161
• Storage: FLASH antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: FLASH Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: FLASH Antibody: CED-4, FLASH, RIP25, KIAA1315, CAS
• Note: For research use only
• Application notes: FLASH antibody can be used for detection of FLASH by Western blot at 0.5 μg/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 µg/mL.

Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 µg/mL.

Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 µg/mL.