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CASP3 monoclonal antibody (M02), clone 4D3

Catalog Number: orb2295092

DispatchUsually dispatched within 5-10 working days
$ 700.00
Catalog Numberorb2295092
CategoryAntibodies
DescriptionMouse monoclonal antibody raised against a partial recombinant CASP3.
ClonalityMonoclonal
Species/HostMouse
IsotypeIgG2a Kappa
ConjugationUnconjugated
ReactivityHuman
Buffer/PreservativesIn 1x PBS, pH 7.4
ImmunogenCASP3 (AAH16926.1, 176 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Protein SequenceSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH
Tested applicationsELISA, PLA
Clone Number4D3
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NoteFor research use only
NCBIAAH16926.1
CASP3 monoclonal antibody (M02), clone 4D3

Detection limit for recombinant GST tagged CASP3 is approximately 1 ng/ml as a capture antibody.

CASP3 monoclonal antibody (M02), clone 4D3

Proximity Ligation Analysis of protein-protein interactions between CASP6 and CASP3. HeLa cells were stained with anti-CASP6 rabbit purified polyclonal 1:1200 and anti-CASP3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).