CARS Antibody

• Catalog Number: orb402338
• Category: Antibodies
• Description: CARS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human CARS recombinant protein (Position: D510-Q748).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 85473 MW
• UniProt ID: P49589
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cysteine--tRNA ligase, cytoplasmic;6.1.1.16;Cystei
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-CARS antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CARS using anti-CARS antibody.Lane 1:human HeLa Cells;2:human COLO-320 Cells;3:human A549 Cells;4:human PANC-1 Cells;5:human 22RV1 Cells.

IF analysis of CARS using anti-CARS antibody.CARS was detected in immunocytochemical section of U20S cell.

IHC analysis of CARS using anti-CARS antibody.CARS was detected in paraffin-embedded section of human placenta tissues.