CARMIL2 Antibody

• Catalog Number: orb1520355
• Category: Antibodies
• Description: CARMIL2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1385 and 1435
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 50-100 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CARMIL2
• UniProt ID: Q6F5E8
• NCBI: Q6F5E8.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: F-actin-uncapping protein RLTPR; RGD, leucine-rich
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse CARMIL2 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, MCF-7, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARMIL2 antibody (orb1520355) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human CARMIL2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARMIL2 antibody (orb1520355) used for IP at 1 µg per reaction.