CARM1 Antibody / PRMT4

• Catalog Number: orb1824844
• Category: Antibodies
• Description: CARM1 (coactivator-associated arginine methyltransferase 1), also known as protein arginine N-methyltransferase 4 (PRMT4), is a 585 amino acid nuclear and cytoplasmic protein belonging to the protein arginine N-methyltransferase family. As a protein arginine N-methyltransferase, CARM1 is capable of catalyzing the transfer of methyl groups from S-adenosylmethionine to the guanidino group nitrogen atoms of arginine residues in certain proteins involved in mRNA stability, DNA packaging and transcriptional regulation. The methyltransferase activity of CARM1 has been found to be negatively regulated through phosphorylation at a conserved serine residue. CARM1 acts as a positive regulator for multiple transcription factors and functions as a secondary coactivator through its association with p160 coactivators. CARM1 exists as two alternatively spliced isoforms, and is encoded by a gene that maps to human chromosome 19p13.2.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CARM1/7426
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the CARM1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CARM1 antibody is available for research use only.
• UniProt ID: Q86X55
• Storage: Aliquot the CARM1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free CARM1 antibody (clone CARM1/7426) as confirmation of integrity and purity.

IHC staining of FFPE human prostate carcinoma tissue with CARM1 antibody (clone CARM1/7426). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate tissue with CARM1 antibody (clone CARM1/7426). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.