CARD9 Antibody

• Catalog Number: orb1520262
• Category: Antibodies
• Description: CARD9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 450 and 500
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 50-100 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CARD9
• UniProt ID: Q9H257
• NCBI: Q9H257.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: hCARD9; CANDF2; caspase recruitment domain-contain
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CARD9 by western blot. Samples: Whole cell lysate (50 µg) from SW620 and HT-29 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARD9 antibody (orb1520262) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human CARD9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SW620 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARD9 antibody (orb1520262) used for IP at 1 µg per reaction.