CARD6 Antibody

• Catalog Number: orb1262399
• Category: Antibodies
• Description: CARD6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This CARD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human CARD6.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 116 kDa
• Target: CARD6
• UniProt ID: Q9BX69
• NCBI: Q9BX69
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Caspase recruitment domain-containing protein 6, C
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody. Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. colon section using CARD6 Antibody. Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody. Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysate from WiDr cell line, using CARD6 Antibody at 1:1000.

Western blot analysis of lysates from A549, HepG2 cell line (from left to right), using CARD6 Antibody at 1:1000 at each lane.

Western blot analysis in WiDr cell line lysates (35 ug/lane).

Western blot analysis of CARD6 Antibody in MDA-MB231 cell line lysates (35 ug/lane)