CARD4/NOD1 Antibody

• Catalog Number: orb234340
• Category: Antibodies
• Description: CARD4/NOD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CARD4 recombinant protein (Position: M1-M160). Human CARD4 shares 82% amino acid (aa) sequence identity with mouse CARD4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By HeatImmunocytochemistry, 0.5-1μg/mlWestern blot, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 107691 MW
• UniProt ID: Q9Y239
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Nucleotide-binding oligomerization domain-containi
• Note: For research use only
• Application notes: WB: The detection limit for CARD4 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CARD4 using anti-CARD4 antibody.Lane 1:A549 Cell;2:Rat Cardiac Muscle Tissue.

IHC analysis of CARD4 using anti-CARD4 antibody.CARD4 was detected in paraffin-embedded section of Human Placenta Tissue.

IHC analysis of CARD4 using anti-CARD4 antibody.CARD4 was detected in immunocytochemical section of A549 cell.

IHC analysis of CARD4 using anti-CARD4 antibody.CARD4 was detected in immunocytochemical section of HeLa cell.

IHC analysis of CARD4 using anti-CARD4 antibody.CARD4 was detected in immunocytochemical section of SMMC-7721 cell.