CARD16 Antibody

• Catalog Number: orb1247658
• Category: Antibodies
• Description: CARD16 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-ETLGLSAGPIPGN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CARD16
• UniProt ID: Q5EG05
• NCBI: NP_443121.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CARD16, COP, CARD only protein, PSEUDO-ICE, COP1,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247658 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing mitochondrial staining. The nuclear stain is DAPI.

orb1247658 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).