Carboxypeptidase A antibody (Biotin)

• Catalog Number: orb344953
• Category: Antibodies
• Description: Carboxypeptidase A antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine
• Isotype: IgG
• Immunogen: Carboxypeptidase A [Bovine Pancreas]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:3,000
• Form/Appearance: Lyophilized
• Purity: Anti-Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas]. Cross reactivity against Carboxypeptidase A from other sources may occur but have not been specifically determined.
• Conjugation: Biotin
• UniProt ID: P00730
• NCBI: NP_777175.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Carboxypeptidase A antibody biotin con
• Note: For research use only
• Application notes: Anti-Carboxypeptidase A Biotin Conjugated Antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Carboxypeptidase A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:55,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.