CAP-H2 Antibody

• Catalog Number: orb1526914
• Category: Antibodies
• Description: CAP-H2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 425 and 475
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CAP-H2
• UniProt ID: Q6IBW4
• NCBI: NP_689512.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: hCAP-H2; Non-SMC condensin II complex subunit H2;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CAP-H2 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, Jurkat, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-H2 antibody (orb1526914) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human CAP-H2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAP-H2 antibody (orb1526914) used for IP at 6 µg per reaction. CAP-H2 was also immunoprecipitated by a previous lot of this antibody (orb1526914) and rabbit anti-CAP-H2 antibody Brother.

Detection of human CAP-H2 by immunohistochemistry.Sample: FFPE section of human prostate cancer. Antibody: Affinity purified rabbit anti-CAP-H2 (Cat. No. orb1526914) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB