Canine TNF-alpha Protein

• Catalog Number: orb762406
• Category: Proteins
• Description: Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
• Reactivity: Canine
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 18.6 kDa
• Target: TNF-alpha
• Source: Canine TNF-alpha, His Tag (orb762406) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P51742-1).
• Expression System: HEK293
• Biological Activity: Immobilized Humira at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag with a linear range of 0.3-1 ng/mL (QC tested).
• Expression Region: Val 77 - Leu 233
• Endotoxins: 1.0 EU per μg
• NCBI: NP_001003244.4
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.6 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Humira at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag (Cat. No. orb762406) with a linear range of 0.3-1 ng/mL (QC tested).