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Canine TNF-alpha Protein

Catalog Number: orb762406

DispatchUsually dispatched within 5-10 working days
$ 640.00
Catalog Numberorb762406
CategoryProteins
DescriptionCanine TNF-alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P51742-1).
ReactivityCanine
TagC-6×His
Form/AppearancePowder
Purity90%
ConjugationUnconjugated
MW18.6 kDa
TargetTNF-alpha
UniProt IDP51742
SourceCanine TNF-alpha, His Tag (orb762406) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P51742-1).
Expression SystemHEK293
Biological OriginCanine
Biological ActivityImmobilized Humira at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag with a linear range of 0.3-1 ng/mL (QC tested).
Expression RegionVal 77 - Leu 233
Endotoxins1.0 EU per μg
NCBINP_001003244.4
Storage-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Buffer/PreservativesPBS, pH7.4
Alternative namesDIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
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NoteFor research use only
Application notesThis protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.6 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expiration Date6 months from date of receipt.
Canine TNF-alpha Protein

Immobilized Humira at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag (Cat. No. orb762406) with a linear range of 0.3-1 ng/mL (QC tested).

  • Canine TNFA protein [orb633341]

    ELISA,  WB

    Greater than 95% by SDS-PAGE gel analyses

    17.2 KDa

    E.Coli

    1 mg, 50 μg, 200 μg, 100 μg
  • TNF alpha Protein, Canine, Recombinant [orb1958053]

    98.00%

    17.3 kDa (predicted); 17 kDa (reducing conditions)

    50 μg
  • Canine TNF alpha ELISA Kit [orb1498987]

    Canine

    1 set
  • Canine TNF a Protein [orb428878]

    Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

    Escherichia Coli

    20 μg, 1 mg, 5 μg
  • Canine TNF alpha protein [orb1215698]

    98%

    17.3 kDa

    Yeast

    10 μg