You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb762406 |
---|---|
Category | Proteins |
Description | Canine TNF-alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P51742-1). |
Reactivity | Canine |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 18.6 kDa |
Target | TNF-alpha |
UniProt ID | P51742 |
Source | Canine TNF-alpha, His Tag (orb762406) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P51742-1). |
Expression System | HEK293 |
Biological Origin | Canine |
Biological Activity | Immobilized Humira at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag with a linear range of 0.3-1 ng/mL (QC tested). |
Expression Region | Val 77 - Leu 233 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_001003244.4 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.6 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Humira at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag (Cat. No. orb762406) with a linear range of 0.3-1 ng/mL (QC tested).
ELISA, WB | |
Greater than 95% by SDS-PAGE gel analyses | |
17.2 KDa | |
E.Coli |
98.00% | |
17.3 kDa (predicted); 17 kDa (reducing conditions) |
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | |
Escherichia Coli |