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Catalog Number | orb782628 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SDMA(Symmetric dimethylarginine) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SDMA(Symmetric dimethylarginine). |
Tested applications | ELISA |
Reactivity | Canine |
Range | 0.16-10 ng/mL |
Concentration | 10 ng/mL |
Sensitivity | 0.051 ng/mL |
Protein Length | 2h |
Alternative names | Symmetric dimethylarginine ELISA Kit Read more... |
Note | For research use only |
Application notes | standard: 10 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog SDMA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog SDMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog SDMA in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |