Canine IL-2 R alpha / CD25 Protein

• Catalog Number: orb864198
• Category: Proteins
• Description: Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.
• Reactivity: Canine
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 26.4 kDa
• Target: IL-2 R alpha
• Source: Canine IL-2 R alpha, His Tag (orb864198) is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Gln 237 (Accession # O62802-1).
• Expression System: HEK293
• Biological Activity: Immobilized Canine IL-2 R alpha, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2, Fc, with a linear range of 0.1-10 ng/mL (QC tested).
• Expression Region: Asp 22 - Gln 237
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IL2RA,CD25,p55,IL2-RA,IL-2-RA
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.4 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Canine IL-2 R alpha, His Tag (Cat. No. orb864198) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2, Fc, Avitag with a linear range of 0.1-10 ng/mL (QC tested).