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Catalog Number | orb80035 |
---|---|
Category | Proteins |
Description | GMCSF k9 Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14.2 kDa. GMCSF is purified by proprietary chromatographic techniques. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 96.0% as determined by1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE. |
Conjugation | Unconjugated |
Target | k9GM CSF |
Solubility (25°C) | It is recommended to reconstitute the lyophilized GMCSF in sterile deionized water not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPEGPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQNINFKSFKENL KDFLFNIPFD CWKPVKK. |
Biological Origin | E.coli |
Biological Activity | The ED50 as calculated by the dose-dependant stimulation of the proliferation of human TF1 erythroleukemic cells is typically 1-4 ng/ml. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | GMCSF was lyophilized after extensive dialysis against 1x PBS pH= 7.4 +/-0.1. |
Alternative names | CSF-2 protein, MGI-1GM protein, GM-CSF protein, Pl Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |