Canine ACTH(Adrenocorticotropic Hormone) ELISA Kit

• Catalog Number: orb1946476
• Category: Assays and Kits
• Description: Canine ACTH(Adrenocorticotropic Hormone) ELISA Kit
• Reactivity: Canine
• Range: 15.63-1000 pg/mL
• Target: ACTH
• Sensitivity: 4.75 pg/mL
• Alternative names: Corticotropin; Adrenocorticotropin
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Adrenocorticotropic Hormone(ACTH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adrenocorticotropic Hormone(ACTH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adrenocorticotropic Hormone(ACTH) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: Serum, plasma and other biological fluids.
• Expiration Date: Please enquire.