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Catalog Number | orb607815 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to Calpain 1 |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CAPN1/1530 |
Tested applications | ELISA, IHC |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Recombinant full-length human CAPN1 protein |
Concentration | Purified Ab with BSA and Azide at 200ug/ml |
Dilution range | ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined. |
Form/Appearance | 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Conjugation | Unconjugated |
MW | ~80kDa |
Target | CAPN1 |
Entrez | 823 |
UniProt ID | P07384 |
Expression System | CHO cells |
Storage | Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
Buffer/Preservatives | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Alternative names | Calcium dependent protease small subunit 1; Calciu Read more... |
Note | For research use only |
Application notes | Positive Control: HeLa cells. Skin or Tonsil |
Expiration Date | 12 months from date of receipt. |