Calnexin antibody

• Catalog Number: orb20292
• Category: Antibodies
• Description: Goat polyclonal antibody to Calnexin
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:128000, WB: 0.1-1μg/ml, IHC-P: 5μg/ml, IF/ICC: 10μg/ml
• Conjugation: Unconjugated
• MW: 67.6
• Target: Calnexin
• Entrez: 821
• Protein Sequence: SKTPELNLDQFHDKT
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Calnexin antibody, anti CANX antibody, anti C
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 100kDa band observed in Mouse Heart, Human Brain (Cerebellum), NIH3T3 and HeLa lysates (calculated MW of 67.6kDa according to Human NP_001737.1 and 67.2kDa according to Mouse NP_001103969.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7;271(23):13691-6., PMID: 8662691). Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20292 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.

orb20292 (6µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb20292 Immunofluorescence analysis of paraformaldehyde fixed LNCaP cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and membrane staining.

orb20292 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and cytoplasmic staining.

orb20292 (0.5µg/ml) staining of LNCaP (A) and U251 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

orb20292 (0.3µg/ml) staining of Human Cerebellum (A) and (0.5ug/ml) Rat Brain (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Western blot analysis of Human Cerebellum (A) and Colorectal cancer (B) lysate using Calnexin antibody

Western blot analysis of CaCo-2 (A) and NIH3T3 (B) cell lysate using Calnexin antibody

Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells using Calnexin antibody.

Immunohistochemical staining of Human Testis using Calnexin antibody