Caldesmon/CALD1 Antibody

• Catalog Number: orb371656
• Category: Antibodies
• Description: Caldesmon/CALD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Caldesmon recombinant protein (Position: M1-E120). Human Caldesmon shares 87.6% amino acid (aa) sequence identity with rat Caldesmon.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 93231 MW
• UniProt ID: Q05682
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Caldesmon;CDM;CALD1;CAD, CDM;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Caldesmon using anti-Caldesmon antibody.Lane 1:human U20S cell; 2:human A549 cell; 3:human HepG2 cell; 4:human HeLa cell; 5:human CACO-2 cell; 6:mouse NIH/3T3 cell.

IHC analysis of Caldesmon using anti-Caldesmon antibody. Caldesmon was detected in a paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of Caldesmon using anti-Caldesmon antibody. Caldesmon was detected in a paraffin-embedded section of rat intestine tissue.

IHC analysis of Caldesmon using anti-Caldesmon antibody. Caldesmon was detected in a paraffin-embedded section of mouse intestine tissue.