Calcium Sensing R (phospho-T888) antibody

• Catalog Number: orb213666
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CASR
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T888 of human Calcium Sensing Receptor protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CASR
• Entrez: 846, 24247, 12374
• UniProt ID: P41180, Q9QY96, P48442
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti GPRC2A antibody, anti PCAR1 antibody, anti Ex
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Calcium Sensing Receptor (Phospho-T888) expression in MCF7 (A), MDCK (B), U2OS (C), SP2/0 (D), PC12 (E), rat brain (F) whole cell lysates. (Predicted band size: 120 kD; Observed band size: 140 kD)

Immunofluorescent analysis of Calcium Sensing Receptor (Phospho-T888) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.