Calcitonin Antibody / CALCA / CGRP

• Catalog Number: orb1826116
• Category: Antibodies
• Description: Calcitonin is a 32 amino acid polypeptide hormone that preserves skeletal integrity and reduces blood calcium levels by decreasing osteoclast activity in bones, calcium and phosphate reabsorption by kidney tubules and calcium absorption by the intestines. The secretion of Calcitonin from the thyroid is regulated in part by estrogen, which increases Calcitonin mRNA levels. The Calcitonin gene, CALCA, undergoes tissue-specific RNA alternative splicing, resulting in the production of different mRNA transcripts. One transcript encodes procalcitonin as well as both calcium-lowering processed active polypeptides, Calcitonin and katacalcin. An alternative transcript of CALCA encodes the precursor for the neuropeptide referred to as Calcitonin generelated peptide 1, also designated CGRP1 or Alpha-CGRP. CGRP is a widely distributed vasodilatory peptide. Calcitonin and katacalcin are produced primarily in the thyroid, while CGRP is produced in neuronal cells. A second CGRP related gene, CALCB, thought to be derived from an gene duplication event, has been identified in mouse, rat and human. Unlike CALCA, CALCB is not subject to alternative splicing and encodes a single transcript designated CGRP2 or Beta-CGRP. Mature CGRP1 and CGRP2 share significant sequence identity at the protein level differing by only 1-3 amino acid residues, depending on the species.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CALCA/8639R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant fragment of human Calcitonin protein (around aa 1-141) was used as the immunogen for the Calcitonin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1:10-1:20 for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Calcitonin antibody is available for research use only.
• UniProt ID: P01258
• Storage: Aliquot the Calcitonin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human thyroid tissue with Calcitonin antibody (clone CALCA/8639R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human medullary carcinoma tissue with Calcitonin antibody (clone CALCA/8639R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.